منابع مشابه
Ribonucleic Acid Composition of Rat Liver Tumor
Studies on normal tissues have suggested that the ribonucleic acid (RNA) of nuclei, mitochon dna, microsomes, and supernatant fluid isolated from tissue homogenate has a characteristic nu cleotide composition (7, 8, 16), while other data did not support such a view (6). Results obtained in this laboratory revealed, in fact, the occurrence of characteristic ribonucleic acids in rat liver frac ti...
متن کاملStudies on the Estimation of Deoxyribonucleic Acid and Ribonucleic Acid in Rat Brain
A method for the estimation of DNA and RNA in rat brain is described. The principal modifications of preexisting methods involve initial lipid extraction with chloroformmethanol and continuous stirring during alkaline hydrolysis of RNA and acid extraction of DNA. Comparison of ultraviolet-absorptive methods, phosphate determination, and orcinol reaction for RNA, shows a greater degree of corres...
متن کاملTurnover of protein and ribonucleic acid in synaptic subcellular fractions from rat brain.
The time courses of the radioactive labels in subcellular fractions from cerebral cortex of mature rats following intraventricular injection of I%-orotate and 3H-leucine were followed from 4 hours to 12 weeks. Special attention was directed to fractions obtained from nerve endings by sucrose gradient centrifugation. After initial transients, the declines in radioactive labels usually followed f...
متن کاملThe biosynthesis of transfer ribonucleic acid in the developing rat brain and in cultured glial cells.
The biosynthesis of tRNA was investigated in cultured astroglial cells and the 3-day-old rat brain in vivo. In the culture system astrocytes were grown for 19 days and were then exposed to [3H]guanosine for 1.5-7.5 h; 3-day-old rats were injected with [3H]guanosine and were killed 5-45 min later. [3H]tRNA was extracted, partially purified, and hydrolyzed to yield [3H]guanine and [3H]methyl guan...
متن کاملLocalization of deoxyribonucleic acid complementary to ribosomal ribonucleic acid and preribosomal ribonucleic acid in the nucleolus of rat liver.
The fraction and distribution of DNA complementary to ribosomal RNA and preribosomal RNA was investigated by the technique of molecular hybridization with the DNA of isolated nucleoli and nuclei of rat liver. Nuclear DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.014% and 0.032%, respectively. Nucleolar DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.10% and 0.22%, respec...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1090533